Prokaryotic Ubiquitin-Like Protein Modification
نویسندگان
چکیده
منابع مشابه
"Depupylation" of prokaryotic ubiquitin-like protein from mycobacterial proteasome substrates.
Ubiquitin (Ub) provides the recognition and specificity required to deliver proteins to the eukaryotic proteasome for destruction. Prokaryotic ubiquitin-like protein (Pup) is functionally analogous to Ub in Mycobacterium tuberculosis (Mtb), as it dooms proteins to the Mtb proteasome. Studies suggest that Pup and Ub do not share similar mechanisms of activation and conjugation to target proteins...
متن کاملDop functions as a depupylase in the prokaryotic ubiquitin-like modification pathway.
Post-translational modification of proteins with prokaryotic ubiquitin-like protein (Pup) is the bacterial equivalent of ubiquitination in eukaryotes. Mycobacterial pupylation is a two-step process in which the carboxy-terminal glutamine of Pup is first deamidated by Dop (deamidase of Pup) before ligation of the generated γ-carboxylate to substrate lysines by the Pup ligase PafA. In this study,...
متن کاملProteasomal protein degradation in Mycobacteria is dependent upon a prokaryotic ubiquitin-like protein.
The striking identification of an apparent proteasome core in Mycobacteria and allied actinomycetes suggested that additional elements of this otherwise strictly eukaryotic system for regulated protein degradation might be conserved. The genes encoding this prokaryotic proteasome are clustered in an operon with a short open reading frame that encodes a small protein of 64 amino acids resembling...
متن کاملAllosteric transitions direct protein tagging by PafA, the prokaryotic ubiquitin-like protein (Pup) ligase.
Protein degradation via prokaryotic ubiquitin-like protein (Pup) tagging is conserved in bacteria belonging to the phyla Actinobacteria and Nitrospira. The physiological role of this novel proteolytic pathway is not yet clear, although in Mycobacterium tuberculosis, the world's most threatening bacterial pathogen, Pup tagging is important for virulence. PafA, the Pup ligase, couples ATP hydroly...
متن کاملPup, a prokaryotic ubiquitin-like protein, is an intrinsically disordered protein.
Pup (prokaryotic ubiquitin-like protein) from Mycobacterium tuberculosis is the first ubiquitin-like protein identified in non-eukaryotic cells. Although different ubiquitin-like proteins from eukaryotes share low sequence similarity, their 3D (three-dimensional) structures exhibit highly conserved typical ubiquitin-like folds. Interestingly, our studies reveal that Pup not only shares low sequ...
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ژورنال
عنوان ژورنال: Annual Review of Microbiology
سال: 2014
ISSN: 0066-4227,1545-3251
DOI: 10.1146/annurev-micro-091313-103447